RNDr. Jan Hrbac, Ph. D.

Education, Employment

1990 – 1995
M.Sc. Inorganic chemistry, Palacký University, Olomouc
1995 – 2001
Ph.D. Physical chemistry, Palacký University, Olomouc
2004
RNDr. Physical chemistry, Palacký University, Olomouc
1998 –1999
Research assistant, Department of Inorganic and Physical Chemistry,  Palacky University, Olomouc
1999
Senior lecturer, Department of Physical Chemistry,  Palacky University, Olomouc

Stays abroad

1999 – 2000, June 2005, July 2007

Department of Pharmaceutics , School of Pharmacy, Hebrew University of Jerusalem, Israel (prof. R. Kohen)

November-December 1996, January 1998, April 2007 

National Center for Scientific Research "Demokritos", Athens, Greece (prof. K. Papadopoulos)

Research specialization

Electrochemistry, sensors, chemiluminescence and electrogenerated chemiluminescence, micellar systems, radicals and antioxidants, instrumentation.

Lecture notes

  • Hrbáč J; Základy práce s PC, VUP Olomouc, 2007

Publications

  1. Papadopoulos K, Chantron A, Nikokavouras J, Hrbac J, Lasovsky J: Sensitized chemiluminescence with long alkyl chain energy donors and acceptors in micellar media. J. PHOTOCHEM. PHOTOBIOL. A, 116: 153 1998
  2. Kohen R, Vellaichamy E, Hrbac J, Gati I, Tirosh O; Quantification of the overall reactive oxygen species scavenging capacity of biological fluids and tissues, FREE RADICAL BIO MED 28 (6): 871-879 2000
  3. Hrbac J, Kohen R; Biological redox activity: Its importance, methods for its quantification and implication for health and disease, DRUG DEVELOP RES 50 (3-4): 516-527 2000
  4. Ginsburg I, Sadovnic M, Yedgar S, Kohen R, Hrbac J; Hemolysis of human erythrocytes by hypochlorous acid is modulated by amino acids, antioxidants, oxidants, membrane-perforating agents and by divalent metals FREE RADICAL RES 36 (6): 607-619 2002
  5. Vrba J, Hrbac J, Ulrichova J, Modriansky M; Sanguinarine is a potent inhibitor of oxidative burst in DMSO-differentiated HL-60 cells by a non-redox mechanism CHEM-BIOL INTERACT 147 (1): 35-47 2004
  6. Hrbac J, Sichertova D, Bancirova M, et al. Sensitized chemiluminescence in micellar mixtures of phthalhydrazide and selected dyes JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY 167 (2-3): 169-175 2004
  7. Frebortova J, Fraaije MW, Galuszka P, Sebela M, Pec P, Hrbac J, Novak O, Bilyeu KD, English JT, Frebort I: Catalytic reaction of cytokinin dehydrogenase: Preference for quinones as electron acceptors BIOCHEM J 380(Pt 1): 121-130 2004
  8. Psotova J, Chlopcikova S, Miketova P, et al. Chemoprotective effect of plant phenolics against anthracycline-induced toxicity on rat cardiomyocytes. Part III. Apigenin, baicalelin, kaempherol, luteolin and quercetin PHYTOTHERAPY RESEARCH 18 (7): 516-521 2004
  9. Kvítek L, Prucek R, Panáček A, Novotný R, Hrbáč J, Zbořil R; The influence of complexing agent concentration on particle size in the process of SERS active silver colloid synthesis. JOURNAL OF MATERIALS CHEMISTRY 15 (10): 1099-1105 2005
  10. Hrbáč J, Broncová G, Matějka P, Bystroň T, Vrba J, Volf R, Vičar J; Electrochemistry of benzophenanthridine alkaloids. Formation and characterization of redox active films from products of sanguinarine and chelerythrine oxidation. ELECTROANALYSIS 17 (23): 2175-2181 2005
  11. Psotova J, Vecera R, Zdarilova A, Anzenbacherova E, Kosina P, Svobodova A, Hrbac J, Jirovsky D, Stiborova M, Lichnovsky V, Vicar J, Simanek V, Ulrichova J, Safety assessment of sanguiritrin, alkaloid fraction of Macleaya cordata, in rats, VETERINARNI MEDICINA 51 (4): 145-155 2006
  12. Komrskova D, Lojek A, Hrbac J, Ciz M.; A comparison of chemical systems for luminometric determination of antioxidant capacity towards individual reactive oxygen species Luminescence 21(4),239-244 2006 (PubMed)
  13. Hrbac J, Zeleny T, Tur'yan YA; Mechanism of the catalyzed by cysteine electroreduction of the insoluble in water cobalt(II) salt as a component of carbon paste electrode J. Solid State Electrochem. 11(6), 841-846, 2007 (Springer)
  14. Hrbac J, Gregor C, Machova M, Kralova J, Bystron T, Ciz M, Lojek A; Nitric oxide sensor based on carbon fiber covered with nickel porphyrin layer deposited using optimized electropolymerization procedure. Bioelectrochemistry, 71(1), 46-53, 2007 (ScienceDirect)
  15. Hrbac J, Halouzka V, Zboril R, Papadopoulos K, Triantis T; Carbon Electrodes modified by Nanoscopic Iron (III) Oxides to assemble chemical sensors for the Hydrogen Peroxide amperometric detection. Electroanalysis 19(17), 1850-1854, 2007. (Wiley InterScience)
  16. Lasovsky J, Hrbac J, Sichertova D, Bednar P; Oxidation and chemiluminescence of catechol by hydrogen peroxide in the presence of Co(II)-ions and CTAB micelles. Luminescence 22(5), 501-506, 2007 (Wiley InterScience)
  17. Trouillas P, Marsal P, Svobodova A, Vostalova J, Gazak R, Hrbac J, Sedmera P, Kren V, Lazzaroni R, Duroux JL, Walterova D; Mechanism of The Antioxidant Action of Silybin and 2,3-Dehydrosilybin Flavonolignans: A Joint Experimental and Theoretical Study. J Phys Chem A, 112 (5), 1054-1063, 2008
  18. Triantis TM, Papadopoulos K, Yannakopoulou E, Dimotikali D, Hrbáč J, Zbořil R; Sensitized chemiluminescence of luminol catalyzed by colloidal dispertions of nanometer-sized ferric oxides. Chemical Engineering Journal, 144 (3):483-488 (2008).
  19. Zdarilova A, Vrublova E, Vostalova J, Klejdus B, Stejskal D, Proskova J, Kosina P, Svobodova A, Vecera R, Hrbac J, Cernochova , Vicar J, Ulrichova J, Simanek V; Natural feed additive of Macleaya cordata: Safety assessment in rats a 90-day feeding experiment. Food and Chemical Toxicology, accepted 2008.